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LC-100 Determination of the active ingredients in Panax

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LC-100 Determination of the active ingredients in Panax

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LC-100 Determination of the active ingredients in Panax notoginseng


                                        
Method: This method uses octadecylsilane bonded silica gel column, UV detector, external standard of each component in Sanqi.


(Edition 2010 Pharmacopoeia)


Assay components: Notoginsenoside R1 ginsenoside Rb1 ginsenosides Rg1.
Test conditions: acetonitrile and water as the mobile phase gradient elution was carried out in accordance with procedures. Detection wavelength 203nm.

          Gradient program:

时间(分钟)

流动相 A(%)

流动相 B(%)

0~12

19

81

12~60

19→36

81→64

 

 

 

 

Spectral map of the tested group

 

                                              对照溶液

 

 

1. notoginsenoside R1 2. ginsenosides Rg1 3. ginsenoside Rb1

 

 

 

 

 

 

 

                                          三七样品